Ácido linoleico conjugado como potencial bioativo para modulação e criotolerância de gametas e embriões

Danielle Storino Freitas; Guilherme Antonio de Gouvêa Lopes; Barbara Rodrigues Nascimento; Luiza Aparecida Ansaloni Chagas Pereira; Ribrio Ivan Tavares Pereira Batista; Paulo Henrique Almeida Campos Junior

doi:10.1590/1809-6891v21e-63574

Autores

Danielle Storino Freitas Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0002-2336-518X
Guilherme Antonio de Gouvêa Lopes Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0002-8097-3237
Barbara Rodrigues Nascimento Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0002-4579-0662
Luiza Aparecida Ansaloni Chagas Pereira Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0001-5767-4936
Ribrio Ivan Tavares Pereira Batista Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0003-4938-2655
Paulo Henrique Almeida Campos Junior Universidade Federal de São João del-Rei (UFSF), São João del-Rei, Minas Gerais, Brasil https://orcid.org/0000-0003-2400-2548

DOI:

https://doi.org/10.1590/1809-6891v21e-63574

Resumo

O ácido linoleico conjugado (CLA) é uma mistura de isômeros posicionais do ácido linoleico encontrado em carne e laticínios de ruminantes. É um tipo de gordura trans muito utilizada por atletas para como suplemento alimentar devido a um suposto efeito de maximizar a utilização das reservas de gordura corporal. O interesse na suplementação de dietas e meios de cultura com o CLA é uma área emergente, exigindo estudos para elucidar seus benefícios nos parâmetros reprodutivos e na criopreservação. Dessa forma, o objetivo dessa revisão foi discutir os efeitos do CLA na criotolerância de oócitos, espermatozóides e embriões. Alguns estudos já demonstraram seu uso na criopreservação da linhagem germinativa. Entre esses, foi observado que a suplementação de CLA durante a maturação in vitro de oócitos pode aumentar sua viabilidade pós-congelamento e capacidade de desenvolvimento. Em relação ao uso de CLA no esperma, existem poucos estudos e seus resultados ainda são inconclusivos. Por último, estudos sobre a suplementação de CLA em meios de cultura de embriões mostraram resultados promissores, indicando que essa molécula bioativa é capaz de modular a captação de lipídios em blastômeros. No total, essas descobertas demonstram o potencial uso do CLA como uma molécula bioativa para melhorar a linha germinativa e a criotolerância ao embrião e abrir novas perspectivas no campo da reprodução humana e animal.
Palavras-chave: acúmulo lipídico, criopreservação, embrião, oócito, sêmen.

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