Ação dos crioprotetores glicose, trealose e quitosana na manutenção da viabilidade de células de Escherichia coli, Saccharomyces cerevisiae após liofilização

Andressa Barella de Freitas; Creciana Maria Endres; Diane Martini; Andréia Paula Dal Castel

doi:10.1590/1809-6891v21e-47464

Autores

Andressa Barella de Freitas Senai https://orcid.org/0000-0003-4713-974X
Creciana Maria Endres SENAI https://orcid.org/0000-0002-5662-2197
Diane Martini SENAI https://orcid.org/0000-0002-2564-6428
Andréia Paula Dal Castel SENAI https://orcid.org/0000-0003-1221-8843

DOI:

https://doi.org/10.1590/1809-6891v21e-47464

Resumo

Resumo
A liofilização é considerada uma das técnicas mais seguras para obtenção de estabilidade de cepas de cultura. Para este processo, podem ser utilizados crioprotetores, que são substâncias que protegem as estruturas celulares durante o período de congelamento, descongelamento e desidratação. O objetivo do presente trabalho foi avaliar o desempenho da glicose, trealose e quitosana como crioprotetores para manutenção de cepas de Escherichia coli e Saccharomyces cerevisiae. Para tratamento estatístico, utilizou-se ANOVA e teste de Tukey com o software Bioestat, versão 5.3. Para Escherichia coli, a Trealose apresentou melhores resultados após a liofilização, porém nenhum dos tratamentos mostrou-se eficaz em prolongar a viabilidade até os 60 dias de armazenamento. Para Saccharomyces cerevisiae, todos os tratamentos apresentaram-se satisfatórios ao longo dos 60 dias avaliados.
Palavras-chave: Conservação; estabilidade de cepas; liofilização; microrganismos.

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