Amplificação cruzada e padronização de marcadores microssatélites em Colossoma macropomum (tambaqui)

Authors

  • Jacqueline Souza Lima Universidade Federal de Goiás
  • Mariana Pires de Campos Telles Universidade Federal de Goiás
  • Lucileide Vilela Resende Universidade Federal de Goiás
  • Felipe Oliveira Gouveia Universidade Federal de Goiás
  • Talge Aiex Boni Universidade Federal de Goiás
  • Ana Christina Sanches Universidade Católica de Goiás

DOI:

https://doi.org/10.5216/rbn.v5i2.9821

Keywords:

Genetic markers, fish, transferability

Abstract

Colossoma macropomum (tambaqui) is a widely grown species in fish farms troughout Brazil, and is already showing genetic variability loss in some stocks. This study aimed at testing the crossamplification in this species based on 52 microsatellite loci originally developed for other fish species. Tambaqui individuals were collected in fish farms in Goiânia, Brazil, and the DNA for amplification was extracted from muscle tissue. The cross-amplification tests were performed changing the temperature of each locus, searching for amplification standardization. Among the 52 tested loci, 16 showed satisfactory cross-amplification results, which allowed the definition of genotypes. These results indicate the existence of a great potential for microsatellite markers transferability among different fish species. These markers can be used in population-genetic studies of this species in conservation programs, management, and improvement of this natural resource.

Downloads

Download data is not yet available.

Author Biographies

Jacqueline Souza Lima, Universidade Federal de Goiás

Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de
Goiás, Campus Samambaia

Ana Christina Sanches, Universidade Católica de Goiás

Departamento de Zootecnia, Universidade Católica de Goiás, Campus II

References

Araújo, L. C. & M. Gouding. 1998. Os frutos do tambaqui: ecologia, conservação e cultivo na Amazônia. Sociedade Civil Mamirauá/CNPq, Brasília, DF, 186 p.

Barbosa, A. C. D. R., T. C. Corrêa, F. Galzerani, P. M. Galetti-Jr. & T. Hatakana. 2006. Thirteen polymorphic microsatellite loci in Neotropical fish Prochilodus argenteus (Characiformes, Prochilodontidae). Mol. Ecol. Notes 6: 936-938.

Brondani, C., P. H. N. Rangel, T. C. O. Borba & R. P. V. Brondani. 2003. Transferability of microsatellite and sequence tagged site markers in Oryza species. Hereditas 138: 187-192.

Calcagnotto, D., M. Russello & R. De-Salle. 2001. Isolation and characterization of microsatellite loci in Piaractus mesopotamicus and their applicability in other Serrasalminae fish. Mol. Ecol. Notes 1: 245-247.

Calcagnotto, D. & S. A. Toledo-Filho. 2000. Loss of genetic variability at the transferrin locus in five hatchery stocks of tambaqui (Colossoma macropomum). Genet. Mol. Biol. 23: 127-130.

Carreras-Carbonell, J., E. MacPherson & M. Pascual. 2007. Utility of pairwise mtDNA genetic distances for predicting cross species microsatellite amplification and polymorphism success in fishes. Conserv. Genet. 9: 181-190.

Carvalho-Costa, L. F., T. Hatakana & P. M. Galetti-Jr. 2006. Isolation and characterization of polymorphic microsatellite markers in the migratory freshwater fish Prochilodus costatus. Mol. Ecol. Notes 6: 818-819.

Creste, S., A. Tulmann Neto & A. Figueira. 2001. Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol. Biol. Rep. 19: 299-306.

Gao, L. Z., C. H. Zhang & J. Z. Jia. 2005. Crossspecies transferability of rice microsatelites in its wild relatives and the potential for conservation genetic studies. Genet. Resour. Crop Evol. 52: 931-940.

Grattapaglia, D., R. P. V. Brondani & C. Brondani. 2008. Manual prático para desenvolvimento de marcadores microssatélites em plantas. Embrapa, Brasília, DF.

Gupta, P. K. & R. K. Varshney. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113: 163-185.

Hatanaka, T., F. Henrique-Silva & P. M. Galetti-Jr. 2006. Population substructuring in a migratory freshwater fish Prochilodus argenteus (Characiformes, Prochilodontidae) from the São Francisco River. Genética 126: 153-159.

IBAMA. Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis. Ministério da Agricultura, Pecuária e Abastecimento, Secretaria Especial de Aqüicultura e Pesca. 2005. Produção brasileira de aqüicultura continental. Brasília, DF.

Katzir, N., Y. Danin-Poleg, G. Tzuri, Z. Karchi, U. Lavi & P. B. Cregan. 1996. Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor. Appl. Genet. 93: 1282-1290.

Liao, X., D. Wang, X. Yu, W. Li, L. Cheng, J. Wang & J. Tong. 2007. Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae. Conserv. Genet. 8: 1003-1007.

Magalhães, A. L. B., R. F. Andrade, T. F. Ratton & M. F. G. Brito. 2002. Ocorrência da truta arco-íris Oncorhynchus mykiss (Walbaum, 1792) (Pisces: Salmonidae) no alto rio Aiuruóca e tributários, bacia do rio Grande, Minas Gerais, Brasil. Bol. Museu Biol. Mello Leitão 14: 35-42.

Matsuoka, Y., S. E. Mitchell & S. Kresovich, M. Goodman & J. Doebley. 2002. Microsatellites in Zea-variability, patterns of mutations, and use for evolutionary studies. Theor. Appl. Genet. 104: 436-450.

Morelli, K. A., E. Revaldaves, C. Oliveira & F. Foresti. 2007. Isolation and characterization of eight microsatellite loci in Leporinus macrocephalus (Characiformes: Anostomidae) and cross-species amplification. Mol. Ecol. Notes 7: 32-34.

Moretzohn, M. C. 2006. Desenvolvimento e mapeamento de marcadores microssatélites e identificação de QTLs ligados à produtividade e à resistência à mancha preta em Arachis sp. Tese de Doutorado. Universidade de Brasília, Brasília, DF.

Paterson, I. G., E. Crispo, M. T. Kinnison, A. P. Hendry & P. Bentzen. 2005. Characterization of tetranucleotide microsatellite markers in guppy (Poecilia reticulata). Mol. Ecol. Notes 5: 269-271.

Plieske, J. & D. Struss. 2001. Microsatellite markers for genome analysis in Brassica L. development in Brassica napus and abundance in Brassicaceae species. Theor. Appl. Genet. 102: 689-694.

Saha, C. M., J. D. Cooper, M. A. Rouf Mian, K. Chekhovskiy & G. D. May Theor. 2006. Tall fescue genomic SSR markers: development and transferability across multiple grass species. Appl. Genet. 113: 1449-1458.

Saracura, V. F. & N. Casagnolli. 1990. Comparação do desempenho entre os alevinos de pacu (Piaractus mesopotamicus) e híbridos de pacu e tambaqui (Colossoma macropomum). Ciênc. Zootéc. 5: 17-19.

Simon, J. C., S. Baumann, P. Sunnucks, P. D. N. Hebert, J. S. Pierre Gallic & C. A. Dedryver. 1999. Reproductive mode and population genetic structure of the cereal aphid Sitobion avenae studied using phenotypic and microsatellite markers. Mol. Ecol. 8: 531-545.

Strecker, U. 2003. Polymorphic microsatelites isolated from the cave fish Astyanax fasciatus. Mol. Ecol. Notes 3: 150-151.

Villacorta-Correa, M. A. & U. Saint-Paul. 1999. Structural indexes and sexual maturity of tambaqui Colossoma macropomum (Cuvier, 1818) (Characiformes: characidae) in central Amazon, Brazil. Rev. Bras. Biol. 59: 637-652.

Downloads

Published

2010-05-07

How to Cite

LIMA, J. S.; TELLES, M. P. de C.; RESENDE, L. V.; GOUVEIA, F. O.; BONI, T. A.; SANCHES, A. C. Amplificação cruzada e padronização de marcadores microssatélites em Colossoma macropomum (tambaqui). Revista de Biologia Neotropical / Journal of Neotropical Biology, Goiânia, v. 5, n. 2, p. 41–49, 2010. DOI: 10.5216/rbn.v5i2.9821. Disponível em: https://revistas.ufg.br/RBN/article/view/9821. Acesso em: 13 nov. 2024.

Issue

Vol. 5 No. 2 (2008)

Section

Articles

License

The expontaneos submmition of the manuscript automaticaly implies in the cession of all patrimonial rights for the Journal of Neotropical Bilogy (RBN) after publication. The autor allow the right of first publication of the article to the RBN, under Creative Commons Attribution 4.0 (CC BY-NC 4.0) Licence.

There are garanties for the authors to the authorial and moral rights, for each one of the articles published by RBN, with permissions:

1. The use of article and contents for the education and researches.

2. The use of the article and their contents, linking to the Article on the web site of the RBN, allowing the divulgation on:

  • institutional closed web (intranet).
  • open access repositories.

3. Preparation and divulgation of the other publication derived from the article and its content, if there is citation of the original publication by RBN.

4. Make printed copies in small quatinties for personal use.