Molecular characterization of Coffea arabica L. accessions from Trujillo, Valle del Cauca, Colombia using SSR markers
DOI:
https://doi.org/10.5216/rbn.v21i1.78154Keywords:
artificial selection, genetic diversity, genetic structure, microsatellitesAbstract
Coffea arabica is one of the most economically important species. It produces one of the most consumed beverages in the world. In this study, with the purpose of using the results to guide improvement plans and promote regional coffee production, some of the coffee varieties grown in the department of Valle del Cauca were molecularly evaluated. A total of 140 individuals corresponding to five coffee varieties were sampled. Eight microsatellite markers were used for molecular characterization based on genetic diversity estimators, a dendrogram based on Nei distances and an AMOVA. The observed mean heterozygosity was found to be very low (0.004), but the expected mean heterozygosity together with the number of haplotypes showed that there is still a high genetic diversity in all varieties despite the breeding programs. This, together with the result of the AMOVA, shows that this diversity is housed in different types of homozygotes, which cause a great deal of variation in the comparison of individuals within each variety. Finally, the Nei distances show a differentiation of the varieties. Those with a higher intensity of artificial selection are more similar.
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