ASCORBIC ACID IN EXTENDER FOR CRYOPRESERVATION OF GOAT SEMEN
DOI:
https://doi.org/10.1590/1809-6891v18e-40117Keywords:
Animal ReproductionAbstract
The objective of this study was to determine the optimal inclusion level of ascorbic acid in TRIS-yolk extenders for cryopreservation of goat semen. We used five ejaculates of three Anglo Nubian goats. Semen from each animal was divided into four 200?L aliquots, composing four treatments: no addition of ascorbic acid (control) and 0.0528, 0.1056, and 0.1584mg/mL of ascorbic acid in TRIS-egg yolk. We evaluated motility and sperm vigor after dilution, post-cooling, and post-thawing; membrane integrity (HOST); acrosomal integrity, and slow thermoresistance test (TRT). Data were subjected to regression analysis at 5% probability. There was no difference for the progressive motility and sperm vigor, respectively, in the post-dilution, after cooling and post-thawing, as well as the additional tests HOST and after 120 minutes of TRT, for motility and force, respectively, in post-thawing (P>0.05). There was a quadratic effect with the addition of ascorbic acid to the acrosome integrity parameter, presenting a great maximum level of 0.1006 mg/mL ascorbic acid for acrosome integrity of 61.58% (P<0.05). The addition of ascorbic acid was effective in maintaining sperm viability, especially acrosome integrity during the cryopreservation process until the level of 0.1006 mg/mL, and it may be an alternative composition of seminal extenders for goats.Keywords: antioxidants; oxidative stress; sperm; TRIS-yolk.
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Published
2017-08-30
How to Cite
BRITO, L. S.; BARBOSA, L. P.; RESENDE, M. V.; PINHEIRO, A. M.; FRANÇA, C. S. da; CARDOSO, R. de C. ASCORBIC ACID IN EXTENDER FOR CRYOPRESERVATION OF GOAT SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 18, 2017. DOI: 10.1590/1809-6891v18e-40117. Disponível em: https://revistas.ufg.br/vet/article/view/e-40117. Acesso em: 5 apr. 2025.
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ZOOTECNIA
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Copyright (c) 2017 CIÊNCIA ANIMAL BRASILEIRA (Brazilian Animal Science)
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