Detection of Escherichia coli O157:H7 and APEC in water samples of  poultry production

Priscila Diniz Lopes; Tatiane Hass; Maria do Ó Mendes da Rosa

doi:10.1590/1809-6891v25e-79310E

Autores

Priscila Diniz Lopes Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0001-9121-8205
Tatiane Hass JBS S. A./ Seara Alimentos, Nuporanga, São Paulo, Brasil https://orcid.org/0009-0009-7377-8968
Maria do Ó Mendes da Rosa JBS S. A./ Seara Alimentos, Nuporanga, São Paulo, Brasil https://orcid.org/0009-0006-1341-9507

DOI:

https://doi.org/10.1590/1809-6891v25e-79310E

Resumo

Escherichia coli são bactérias comensais e estão presentes no ambiente. Entretanto, alguns patotipos podem causar doenças em animais e humanos. E. coli APEC causa a colibacilose em aves, provocando sinais respiratórios e sistêmicos. O sorotipo enterohemorrágico O157:H7 causa infecções transmitidas por alimentos em humanos, provocando quadros de diarreia hemorrágica e síndrome hemolítico-urêmica. O objetivo do estudo foi detectar os sorotipos E. coli APEC e O157:H7 em amostras de água em diferentes pontos da produção avícola e avaliar os perfis de resistência antimicrobiana. A análise de PCR foi realizada para detectar os padrões dos genes hlyF, iutA, iroN, iss e ompT de E. coli APEC e o antígeno flagelar H7 e as toxinas Stx1 e Stx2 de E. coli O157:H7. Trinta e três amostras de água de diferentes pontos foram positivas para E. coli, entre elas, oito para APEC e nenhuma para O157:H7. Os isolados de APEC apresentaram diferentes graus de suscetibilidade aos antimicrobianos.

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