Lactic acid bacteria inhibit Salmonella Heidelberg biofilm  formation on polystyrene surfaces

Luciane Manto; Bruna Webber; Enzo Mistura; Karen Apellanis Borges; Thales Quedi Furian; Jucilene Sena dos Santos; Luciana Ruschel dos Santos

doi:10.1590/1809-6891v25e-76376E

Autores

Luciane Manto Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-6269-2802
Bruna Webber Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-6578-7105
Enzo Mistura Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-9510-0121
Karen Apellanis Borges Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-6649-5833
Thales Quedi Furian Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0003-0376-8616
Jucilene Sena dos Santos Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-9771-7264
Luciana Ruschel dos Santos Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-7813-4795

DOI:

https://doi.org/10.1590/1809-6891v25e-76376E

Resumo

Salmonella spp. é uma das principais causas de gastroenterite em todo o mundo. Salmonella Heidelberg é um patógeno emergente associado com surtos com multirresistência antimicrobiana vinculados aos produtos avícolas. A sua alta persistência no ambiente pode estar associada com sua
habilidade de aderir a diferentes superfícies e formar biofilmes. Devido ao aumento da resistência antimicrobiana em todo o mundo, os pesquisadores têm investigado o uso de bactérias acido láticas (BAL) como um controle biológico e de microrganismos patogênicos. O objetivo deste estudo foi avaliar a habilidade de BAL no controle de biofilmes produzidos por S. Heidelberg em placas de poliestireno. Foi avaliada a atividade antimicrobiana in vitro de nove BAL, todas pertencentes ao gênero Lactobacillus, na inibição e na remoção de biofilmes produzidos por S. Heidelberg. A formação de biofilme só ocorreu quando a BAL1 (Lactobacillus salivaris) foi utilizada. Todos os outros tratamentos demonstraram atividade antimicrobiana. Entretanto, a BAL não foi capaz de reduzir a contagem bacteriana. Os resultados obtidos demonstram que BAL são capazes de prevenir ou retardar a formação de biofilme por S. Heidelberg em superfícies de poliestireno e podem ser utilizadas em estudos in vivo para determinar o seu potencial alternativo no controle deste patógeno na indústria de alimentos.

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