Comparação de sete diferentes RT-qPCR para detecção do virus da  febre aftosa

Antônio Augusto Fonseca Júnior; Mateus Laguardia-Nascimento; Amanda Petrina Scotá Ferreira; Anselmo Vasconcelos Rivetti Júnior; Marcelo Fernandes Camargos; René Ribeiro da Silva

doi:10.1590/1809-6891v25e-76304E

Autores

Antônio Augusto Fonseca Júnior Laboratório Federal de Defesa Agropecuária de Minas Gerais (LFDA/MG), Pedro Leopoldo, Minas Gerais, Brasil https://orcid.org/0000-0002-2962-5616
Mateus Laguardia-Nascimento Laboratório Federal de Defesa Agropecuária de Minas Gerais (LFDA/MG), Pedro Leopoldo, Minas Gerais, Brasil https://orcid.org/0000-0003-4428-0764
Amanda Petrina Scotá Ferreira Laboratório Federal de Defesa Agropecuária de Minas Gerais (LFDA/MG), Pedro Leopoldo, Minas Gerais, Brasil https://orcid.org/0000-0002-4105-8052
Anselmo Vasconcelos Rivetti Júnior Laboratório Federal de Defesa Agropecuária de Minas Gerais (LFDA/MG), Pedro Leopoldo, Minas Gerais, Brasil https://orcid.org/0000-0003-2988-2652
Marcelo Fernandes Camargos Laboratório Federal de Defesa Agropecuária de Minas Gerais (LFDA/MG), Pedro Leopoldo, Minas Gerais, Brasil https://orcid.org/0000-0001-8457-7875
René Ribeiro da Silva Laboratório Federal de Defesa Agropecuária do Pará, Belém, Pará, Brasil https://orcid.org/0000-0002-3943-3757

DOI:

https://doi.org/10.1590/1809-6891v25e-76304E

Resumo

O diagnóstico rápido e preciso de surtos de febre aftosa é crucial para conter a rápida transmissão de seu agente causador, o vírus da febre aftosa (FMDV), que representa uma ameaça significativa ao gado do país. Este estudo teve como objetivo realizar uma análise comparativa de vários métodos de RT-qPCR para a detecção do FMDV. Os métodos avaliados para a região 3D demonstraram sensibilidade e especificidade semelhantes. No entanto, foram observadas diferenças notáveis em amostras com baixa concentração de RNA. Embora esses métodos possam ser utilizados de forma intercambiável com resultados consistentes ao lidar com um grande número de amostras enviadas ao laboratório para identificar um surto, variações sutis podem assumir importância ao trabalhar com uma quantidade limitada de amostras contendo baixas cópias de RNA

Downloads

Não há dados estatísticos.

Referências

Alexandersen S, Zhang Z, Donaldson AI, Garland AJ. The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol. 2003 Jul;129(1):1-36. doi: 10.1016/s0021-9975(03)00041-0.

Grubman MJ, Baxt B. Foot-and-mouth disease. Clin Microbiol Rev. 2004 Apr;17(2):465-93. doi: 10.1128/CMR.17.2.465-493.2004.

Amaral TB, Gond V, Tran A (2016) Mapping the likelihood of foot-and-mouth disease introduction along the border between Brazil and Paraguay. Pesquisa Agropecuária Brasileira. 2016; 51(05):661-670. https://doi.org/10.1590/S0100-204X2016000500029

Davies G. The foot and mouth disease (FMD) epidemic in the United Kingdom 2001. Comp Immunol Microbiol Infect Dis. 2002 Oct;25(5-6):331-43. doi: 10.1016/s0147-9571(02)00030-9.

Corbellini LG, Fernández F, Vitale E, Moreira Olmos C, Charbonnier P, Iriarte Barbosa MV, Riet-Correa F. Shifting to foot-and-mouth disease-free status without vaccination: Application of the PROMETHEE method to assist in the development of a foot-and-mouth national program in Uruguay. Prev Vet Med. 2020 Aug;181:105082. doi: 10.1016/j.prevetmed.2020.105082.

OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris. 2018.

Callahan JD, Brown F, Osorio FA, Sur JH, Kramer E, Long GW, Lubroth J, Ellis SJ, Shoulars KS, Gaffney KL, Rock DL, Nelson WM. Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. J Am Vet Med Assoc. 2002 Jun 1;220(11):1636-42. doi: 10.2460/javma.2002.220.1636.

Reid SM, Ferris NP, Hutchings GH, Zhang Z, Belsham GJ, Alexandersen S. Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods. 2002 Aug;105(1):67-80. doi: 10.1016/s0166-0934(02)00081-2. PMID: 12176143.

Oem JK, Kye SJ, Lee KN, Kim YJ, Park JY, Park JH, Joo YS, Song HJ. Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus. J Vet Sci. 2005 Sep;6(3):207-12.

Dill V, Beer M, Hoffmann B. Simple, quick and cost-efficient: A universal RT-PCR and sequencing strategy for genomic characterisation of foot-and-mouth disease viruses. J Virol Methods. 2017 Aug;246:58-64. doi: 10.1016/j.jviromet.2017.04.007.

Goris N, Vandenbussche F, Herr C, Villers J, Van der Stede Y, De Clercq K. Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision. J Virol Methods. 2009 Sep;160(1-2):157-62. doi: 10.1016/j.jviromet.2009.05.005.

El Bagoury GF, Elhabashy R, Mahmoud AH, Hagag NM, El Zowalaty ME. Development and evaluation of one-step real-time RT-PCR assay for improved detection of foot-and-mouth disease virus serotypes circulating in Egypt. J Virol Methods. 2022 Aug;306:114525. doi: 10.1016/j.jviromet.2022.114525.

Foglia EA, Lembo T, Kazwala R, Ekwem D, Shirima G, Grazioli S, Brocchi E, Pezzoni G. Combining Multiple Assays Improves Detection and Serotyping of Foot-and-Mouth Disease Virus. A Practical Example with Field Samples from East Africa. Viruses. 2021 Aug 10;13(8):1583. doi: 10.3390/v13081583. PMID: 34452448; PMCID: PMC8412026.

Shi X, Liu X, Wang Q, Das A, Ma G, Xu L, Sun Q, Peddireddi L, Jia W, Liu Y, Anderson G, Bai J, Shi J. A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses. J Virol Methods. 2016 Oct;236:258-265. doi: 10.1016/j.jviromet.2016.08.005.

Moonen P, Boonstra J, van der Honing RH, Leendertse CB, Jacobs L, Dekker A. Validation of a LightCycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus. J Virol Methods. 2003 Oct;113(1):35-41. doi: 10.1016/s0166-0934(03)00220-9.

Nérette P, Dohoo I, Hammell L, Gagné N, Barbash P, Maclean S, Yason C. Estimation of the repeatability and reproducibility of three diagnostic tests for infectious salmon anaemia virus. J Fish Dis. 2005 Feb;28(2):101-10. doi: 10.1111/j.1365-2761.2005.00613.x. PMID: 15705155.

Moniwa M, Clavijo A, Li M, Collignon B, Kitching PR. Performance of a foot-and-mouth disease virus reverse transcription-polymerase chain reaction with amplification controls between three real-time instruments. J Vet Diagn Invest. 2007 Jan;19(1):9-20. doi: 10.1177/104063870701900103.

Nérette P, Dohoo I, Hammell L, Gagné N, Barbash P, Maclean S, Yason C. Estimation of the repeatability and reproducibility of three diagnostic tests for infectious salmon anaemia virus. J Fish Dis. 2005 Feb;28(2):101-10. doi: 10.1111/j.1365-2761.2005.00613.x. PMID: 15705155.

Hause BM, Collin EA, Anderson J, Hesse RA, Anderson G. Bovine rhinitis viruses are common in U.S. cattle with bovine respiratory disease. PLoS One. 2015 Mar 19;10(3):e0121998. doi: 10.1371/journal.pone.0121998. PMID: 25789939; PMCID: PMC4366061.

El Bagoury GF, Elhabashy R, Mahmoud AH, Hagag NM, El Zowalaty ME. Development and evaluation of one-step real-time RT-PCR assay for improved detection of foot-and-mouth disease virus serotypes circulating in Egypt. J Virol Methods. 2022 Aug;306:114525. doi: 10.1016/j.jviromet.2022.114525. Epub 2022 Mar 23. PMID: 35337855.

Carrillo C, Tulman ER, Delhon G, Lu Z, Carreno A, Vagnozzi A, Kutish GF, Rock DL. Comparative genomics of foot-and-mouth disease virus. J Virol. 2005 May;79(10):6487-504. doi: 10.1128/JVI.79.10.6487-6504.2005.

Stenfeldt C, Eschbaumer M, Rekant SI, Pacheco JM, Smoliga GR, Hartwig EJ, Rodriguez LL, Arzt J. The Foot-and-Mouth Disease Carrier State Divergence in Cattle. J Virol. 2016 Jun 24;90(14):6344-64. doi: 10.1128/JVI.00388-16.

Keck H, Hoffmann B, Eschbaumer M. Proof of Proficiency of Decentralized Foot-and-Mouth Disease Virus Diagnostics in Germany. Viruses. 2022 May 20;14(5):1098. doi: 10.3390/v14051098.

Shi X, Liu X, Wang Q, Das A, Ma G, Xu L, Sun Q, Peddireddi L, Jia W, Liu Y, Anderson G, Bai J, Shi J. A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses. J Virol Methods. 2016 Oct;236:258-265. doi: 10.1016/j.jviromet.2016.08.005.

Xu X, Yang F, Zhang Q, Xu Y, Huang J, Fu M, Zhang W. Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats. J Virol Methods. 2019 Apr;266:58-64. doi: 10.1016/j.jviromet.2019.01.015.

Rasmussen TB, Uttenthal A, de Stricker K, Belák S, Storgaard T. Development of a novel quantitative real-time RT-PCR assay for the simultaneous detection of all serotypes of foot-and-mouth disease virus. Arch Virol. 2003 Oct;148(10):2005-21. doi: 10.1007/s00705-003-0145-2.

Wernike K, Hoffmann B, Beer M. Single-tube multiplexed molecular detection of endemic porcine viruses in combination with background screening for transboundary diseases. J Clin Microbiol. 2013 Mar;51(3):938-44. doi: 10.1128/JCM.02947-12.