Growth and differentiation factor – 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles

André Luiz da Conceição Santos; Anna Clara Ferreira; Naiza Sá; Renato Silva; Gaby Quispe-Palomino; Everton Lopes; Jesús Cadenas; Benner Alves; Juliana Celestino; Ana Paula Rodrigues; José Ricardo Figueiredo

doi:10.1590/1809-6891v24e-74980E

Autores

André Luiz da Conceição Santos Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-5251-9598
Anna Clara Ferreira Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-2125-9471
Naiza Sá Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-4745-0108
Renato Silva Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-0001-2960
Gaby Quispe-Palomino Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-2957-0536
Everton Lopes Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-1250-4818
Jesús Cadenas University Hospital of Copenhagen, Rigshospitalet, Copenhaguen, Denmark https://orcid.org/0000-0001-8164-3697
Benner Alves Universidade Federal de GoiásConception Biosciences, Berkeley, California, United States https://orcid.org/0000-0001-9465-8054
Juliana Celestino Universidade da Integração Internacional da Lusofonia Afro Brasileira (UNILAB), Redenção, Ceará, Brasil https://orcid.org/0000-0002-9930-7541
Ana Paula Rodrigues Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0001-9786-1250
José Ricardo Figueiredo Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-5139-0964

DOI:

https://doi.org/10.1590/1809-6891v24e-74980E

Resumo

Este estudo teve como objetivo investigar o efeito do GDF-9 durante o cultivo in vitro de folículos antrais iniciais caprinos isolados. Os folículos antrais iniciais isolados e selecionados foram cultivados individualmente por 18 dias, e os seguintes tratamentos foram testados: α MEM+ (tratamento controle) ou α-MEM+ suplementado com 200 ng/mL de GDF-9 (tratamento GDF-9). Os seguintes parâmetros foram avaliados: crescimento e morfologia folicular, produção de estradiol, maturação nuclear do oócito e expressão relativa de genes-chave relacionados a esteroidogênese (CYP19A1, CYP17 e receptor de insulina) e remodelamento da membrana basal (MMP-9 e TIMP-2). Em ambos os tratamentos, observou-se diminuição na porcentagem de folículos morfologicamente intactos com aumento concomitante nas taxas de folículos extrusos e degenerados (P < 0,05). O tratamento GDF-9 apresentou maiores taxas de folículos extrusos apenas no 6º dia de cultivo (P < 0,05). O diâmetro do folículo aumentou progressivamente ao longo do período de cultivo (P < 0,05) com diâmetros semelhantes entre os tratamentos em todos os tempos de cultivo (P > 0,05). O GDF-9 aumentou a taxa de crescimento diário do primeiro para o segundo terço de cultivo, sendo maior (P < 0,05) que o controle no segundo terço. A taxa de maturação oocitária assim como os níveis de estradiol e a expressão relativa de RNAm para os genes CYP19A1, CYP17, MMP-9, TIMP-2 e receptor de insulina foram similares entre os tratamentos (P > 0,05). Em conclusão, este estudo mostra pela primeira vez que GDF-9 adicionado a um meio de cultivo aumentou a taxa de crescimento de folículos antrais iniciais caprinos cultivados in vitro.
Palavras-chave: cultivo in vitro; folículo antral; cabra; GDF-9

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