Zebrafish (Danio rerio) meets bioethics: the 10Rs ethical principles in research

Aryelle Canedo; Patrícia Saiki; Andressa Liberal Santos; Karla da Silva Carneiro; Andreza Martins de Souza; Gabriel Qualhato; Rafaella da Silva Brito; Francyelli Mello-Andrade; Thiago Lopes Rocha

doi:10.1590/1809-6891v22e-70884

Autores

Aryelle Canedo Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-4122-3249
Patrícia Saiki Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-7570-6388
Andressa Liberal Santos Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-7541-0790
Karla da Silva Carneiro Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-2458-1386
Andreza Martins de Souza Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-5097-5453
Gabriel Qualhato Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-2593-4745
Rafaella da Silva Brito Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-7685-1811
Francyelli Mello-Andrade Instituto Federal de Goiás (IFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-7389-6125
Thiago Lopes Rocha Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-0551-6842

DOI:

https://doi.org/10.1590/1809-6891v22e-70884

Resumo

O peixe-zebra (Danio rerio) é um peixe tropical amplamente usado em pesquisas em todo o mundo. Devido ao seu uso emergente em várias áreas de pesquisa, o desenvolvimento de animais geneticamente modificados e o aumento das instalações de peixe-zebra, novos desafios éticos surgem na pesquisa com o peixe-zebra. Além disso, é necessária a conscientização da comunidade científica sobre as normas éticas e leis vigentes na pesquisa com animais. Assim, o presente estudo teve como objetivo descrever os princípios éticos de 10 Rs usando o peixe-zebra como sistema modelo em pesquisa. Os 3 Rs clássicos relativos ao bem-estar animal (substituição, redução e refinamento) e 7 Rs adicionais relacionados aos princípios científicos (registro, relatório, robustez, reprodutibilidade e relevância) e de conduta (responsabilidade e respeito) na pesquisa do peixe-zebra são apresentados e discutido criticamente. Recomendamos o uso desses 10 Rs pelos pesquisadores, instituições e Comitê de Ética Animal na regulamentação, decisão e promoção da saúde e bem-estar do peixe-zebra em pesquisas.
Palavras-chave: saúde animal; bem-estar animal; comitês de ética animal; peixe; animais de laboratório.

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