Vancomycin and tetracycline-resistant enterococci from from raw and processed meats: phenotypic and genotypic characteristics of isolates

Luciana Furlaneto Maia; Catia Giraldi; Márcia Regina Terra; Márcia Cristina Furlaneto

doi:10.1590/1809-6891v21e-57674

Autores

Luciana Furlaneto Maia Universidade Tecnológica Federal do Paraná, Londrina, Paraná, Brasil https://orcid.org/0000-0002-7164-3391
Catia Giraldi Universidade Tecnológica Federal do Paraná, Londrina, Paraná, Brasil https://orcid.org/0000-0002-3171-3025
Márcia Regina Terra Universidade Tecnológica Federal do Paraná, Londrina, Paraná, Brasil https://orcid.org/0000-0002-5814-0538
Márcia Cristina Furlaneto Universidade Tecnológica Federal do Paraná, Londrina, Paraná, Brasil https://orcid.org/0000-0002-1188-1320

DOI:

https://doi.org/10.1590/1809-6891v21e-57674

Resumo

A natureza ubíqua dos enterococos e sua capacidade de colonizar diferentes habitats são responsáveis pela sua fácil disseminação pela cadeia alimentar. No presente estudo, avaliamos a distribuição e a susceptibilidade antimicrobiana de isolados de Enterococcus provenientes de produtos cárneos. Cem produtos (carne de frango cru, carne de porco crua e carne cozida) foram adquiridos e cultivados para a presença de Enterococcus spp. No total, 194 amostras foram avaliadas, com taxas de contaminação de 63,6% nas amostras de frango, 31% na carne de porco crua e 1,4% nas amostras de carne cozida. A amplificação por PCR foi realizada para confirmar a presença de Enterococcus spp. (95/96), E. faecalis (66/96), E. faecium (30/96) E. casseliflavus/E. flavescens (3/96). Resultados de susceptibilidade mostraram que 100% dos isolados foram resistentes a pelo menos um antibiótico, sendo 100% de E. faecium resistentes a vancomicina, estreptomicina, ciprofloxacina, norfloxacina, eritromicina e tetraciclina. E. casseliflavus / E. flavescens resistentes a gentamicina, estreptomicina, ciprofloxacina, norfloxacina, eritromicina e tetraciclina. E. faecalis foram resistentes a ciprofloxacina, tetraciclina e eritromicina (92%), norfloxacina (83%), vancomicina e estreptomicina (50%). Na genotipagem, foram detectados os genes tetL e vanB. A presença desses microrganismos resistentes aos antimicrobianos nos alimentos pode causar problemas para a saúde pública.
Palavras-chaves: Antibióticos, enterococci vancomicina-resistante, PCR.

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