Analysis of heat shock transcription factor gene family in cocoa

Authors

  • Waki’atil Rosida
  • Fuad Bahrul Ulum
  • Mukhamad Su’udi Su’udi

Abstract

The heat shock factor (HSF) is crucial for plant stress regulation. This study aimed to analyze the role and characteristics of the HSF protein in Theobroma cacao L. The assesment was carried out bioinformatically, consisting of HSF identification in the cocoa genome, analysis of physical properties, gene structure, motifs, and subcellular, phylogenetics and chromosomal localization. A total of 26 HSF proteins were identified, presenting a complete structure consisting of exons and introns. The analysis of the TcHSF gene promoter revealed several cis-acting elements that act on stress, such as the responsive elements to anaerobiosis, abscisic acid, and low temperature, as well as the myeloblastosis binding sequence. Almost all TcHSF exhibited a strong degree of subcellular localization in the nucleus. Cocoa phylogenetics showed that Thecc1EG037635t1 is in a clade with At5G54070, with a bootstrap value of 90 %. The three best candidates (TcHSF-14, TcHSF-24, and TcHSF-25) are recommended for further functional analysis, due to their potential for increasing cocoa value.

KEYWORDS: Theobroma cacao L., abiotic stress, gene structure.

Downloads

Download data is not yet available.

Downloads

Published

2026-01-07

How to Cite

ROSIDA, Waki’atil; ULUM, Fuad Bahrul; SU’UDI, Mukhamad Su’udi. Analysis of heat shock transcription factor gene family in cocoa. Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics], Goiânia, v. 56, p. e83957, 2026. Disponível em: https://revistas.ufg.br/pat/article/view/83957. Acesso em: 10 jan. 2026.

Issue

v. 56 (2026)

Section

Research Article

License

Authors will not be paid for published articles and must waive their copyright in favor of the PAT journal. On the other hand, they are solely responsible for the content of those articles, even if the Editor holds the right to adjust them to the norms of the journal. Authors are allowed to publish their articles simultaneously in their institutional repositories, as soon as the original publication at the PAT journal is mentioned.