In vitro callus induction in grapevine rootstock

Authors

  • Wendel Adelson Soares da Silva
  • Fernanda Ferreira Salgado
  • Cléber José da Silva
  • José Carlos Moraes Rufini

Abstract

Grapevine (Vitis spp.) is traditionally propagated by cutting and grafting. However, micropropagation represents a promising alternative for the production of plants free of systemic pathogens. This study aimed to evaluate callus induction in grapevine rootstock cv. IAC 766, using the thin cell layer (TCL) technique. Longitudinal (lTCL) and transverse (tTCL) sections were obtained from internodal segments and cultivated in two types of culture media (½ MS and WPM). The experimental design was completely randomized, in a 2 × 2 factorial arrangement, with four replicates per treatment. After 70 days of in vitro culture, the presence of friable or watery calli was evaluated. The WPM medium was superior to ½ MS for the induction of friable calli, with 2 and 0.65 calli formed, respectively. Likewise, the longitudinal section was superior to the transverse one (2.37 and 0.25, respectively).

KEYWORDS: Vitis spp., viticulture, micropropagation.

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Published

2026-01-20

How to Cite

SILVA, Wendel Adelson Soares da; SALGADO, Fernanda Ferreira; SILVA, Cléber José da; RUFINI, José Carlos Moraes. In vitro callus induction in grapevine rootstock. Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics], Goiânia, v. 56, p. e84126, 2026. Disponível em: https://revistas.ufg.br/pat/article/view/84126. Acesso em: 26 mar. 2026.

Issue

v. 56 (2026)

Section

Research Article

License

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