EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS

Authors

  • Cristiane Regina Ramos UFG
  • Edward Madureira Brasil UFG
  • Robson Maia Geraldine UFG

Keywords:

Recovery, aflatoxins, extraction methods, immunoaffinity, HPLC

Abstract

A comparative evaluation process was carried out between two methods of aflatoxins extraction, cleaning, and purification, for High Performance Liquid Chromatography (HPLC) analysis. The first one is the physical-chemical method, based on chemical reactions for all the stages, and the second makes use of a immunoaffinity column (IAC). The methods were evaluated as related to recovery percentage, cost, time spent for analysis, and number of samples analyzed per day. The recovery percentage obtained through the physical-chemical method was lower than the IAC method to B1, B2 and G1 aflatoxins, and higher to G2 aflatoxin. Both techniques presented recovery percentages in accordance with established values by the European Community (70% to 120%). Concerning costs, the physical-chemical method showed to be the least expensive, but its analysis process takes more time, because it involves more steps and demands more caution from laboratory technicians. Despite its higher cost, about six times higher than the physicalchemical method, the IAC method showed to be faster and easier to perform.


KEY-WORDS: Recovery; aflatoxins; extraction methods; immunoaffinity; HPLC.

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Author Biographies

Edward Madureira Brasil, UFG

Universidade Federal de Goiás, Escola de Agronomia e Engenharia de Alimentos. Av. Goiânia-Nova Veneza, km 0, Campus
Samambaia, CEP 74001-970 Goiânia, GO.

Robson Maia Geraldine, UFG

Universidade Federal de Goiás, Escola de Agronomia e Engenharia de Alimentos. Av. Goiânia-Nova Veneza, km 0, Campus
Samambaia, CEP 74001-970 Goiânia, GO.

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Published

2008-06-30

How to Cite

RAMOS, C. R.; BRASIL, E. M.; GERALDINE, R. M. EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS. Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics], Goiânia, v. 38, n. 2, p. 103–108, 2008. Disponível em: https://revistas.ufg.br/pat/article/view/4158. Acesso em: 16 may. 2024.

Issue

v. 38, n. 2, Apr./Jun. 2008

Section

Research Article

License

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