EFFECTS OF ENVIRONMENTAL FACTORS ON THE ACTIVITY OF ACID PHOSPHATASE IN COMMON BEAN

Authors

  • Itamar Pereira de Oliveira Embrapa Arroz e Feijão
  • Michael Djie Thung Centro Internacional de Agricultura Tropical
  • José Renato de Freitas UFG
  • Renato Sérgio Mota dos Santos UFG

Keywords:

Phaseolus vulgaris, temperature, buffered solution, time of reaction

Abstract

Plants with 15 days after the germination were picked in field experiments with the purpose of knowing the best pH, temperature and the necessary time to express the activity of the phosphatase acid in three bean varieties (Phaseolus vulgaris L.), Carioca, EMP-84 and CNF-10, in the presence and in the phosphorus absence. The largest values of activity of the phosphatase acid were observed when the plants were tested in pH 5.5 solution during 120 minutes at the temperature of 30°C. The use of buffer substances as PNPP + Triton X-100 expressed better the activity of the phosphatase acid. The vacuum condition constituted a positive factor to express the activity of the phosphatase acid. The plants developed under drought stress presented smaller activity of the phosphatase acid. The top leaf/root ratio of activity of the phosphatase acid got 5.72 for the Carioca, 4.91 for EMP-84 and 4.36 for CNF-10 variety. The plants when developed under drought stresses condition presented smaller values of activity of the phosphatase acid.

KEY-WORDS: Phaseolus vulgaris; temperature; buffered solution; time of reaction.

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Published

2007-12-19

How to Cite

OLIVEIRA, I. P. de; THUNG, M. D.; FREITAS, J. R. de; SANTOS, R. S. M. dos. EFFECTS OF ENVIRONMENTAL FACTORS ON THE ACTIVITY OF ACID PHOSPHATASE IN COMMON BEAN. Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics], Goiânia, v. 28, n. 2, p. 83–94, 2007. Disponível em: https://revistas.ufg.br/pat/article/view/2999. Acesso em: 17 jul. 2024.

Issue

v. 28, n. 2, Jul./Dec. 1998

Section

Research Article

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