EFFICIENCY OF THE ENRICHMENT BROTHES (LEB1 AND LEB2) AND SELECTIVE AGARS (LPM AND LSAB-CAN) TO ISOLATE BACTERIA OF THE GENUS Listeria IN MEAT AND RESIDUAL WATER OF WASHING CARCASS
Keywords:
Listeria, enrichment broth, selective agarAbstract
In this paper it was observed the efficiency of enrichment brothes for Listeria (LEB1 and LEB2), associated to the passage of the culture through a 0.25% potassium hidroxide solution, verifying that the secondary enrichment broth (LEB2 and LEB2KOH) was better than the primary enrichment broth (LEB1KOH) for this purpose. On the other hand, lithium chloride-phenylethanol-moxalactam agar and the selective Listeria agar base, supplemented with cicloheximide, acriflavine and nalidixic acid (LSAIB-CAN) showed equivalency, at the statistic point of view (p=0.l442), in relation to the number of positive samples for Listeria spp., although the LSAB-CAN agar had given the greatest number of bacteria isolated from this genus.
KEY-WORDS: Listeria; enrichment broth; selective agar.
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