DIRECT ORGANOGENESIS IN Phoenix dactylifera L. THROUGH COTILEDONAR PETIOLE
Keywords:
Date palm, regeneration, growth regulatorsAbstract
There is much interest with respect to the success of the application of tissue culture for propagation of palm family members. Date palm (Phoenix dactylifera L.) is originary from the Middle East, where climate is hot and dry, and due to its botanical characteristics constitutes a good option for cultivation of adapted fruits to Brazilian Northeast. This study had as objective to test 6-Benzilaminopurine (BAP) and indole-3-acetic acid (IAA) growth regulators for date palm micropropagation by direct organogenesis, using Khadrawy cultivar. Cotiledonar petioles were inoculated in MS medium supplemented with four combinations of IAA and BAP, on equal concentrations (0 mg.L-1, 0.5 mg.L-1, 1.0 mg.L-1 and 2.0 mg.L-1). The hormonal concentrations used did not propitiate the multiple buds, but showed relative efficiency in aerial parts and primary roots differentiation, contributing for in vitro conservation strategiesof this species.
KEY-WORDS: Date palm; regeneration; growth regulators.
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