COMPARISON AMONG DECONTAMINATION METHODS AT INITIAL PHASE OF BANANA IN VITRO CULTURE

Authors

  • Iraídes Fernandes Carneiro UFG
  • Lincoln Fonseca Zica UFG
  • Lázaro José Chaves UFG

Keywords:

Banana cv. Maçã, tissue culture, superficial sterilization, Musa AAB

Abstract

Four decontamination methods for in vitro culture of banana (Musa AAB cv. Maçã) explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from axillary buds were submersed in different concentrations of NaOCl for each different period of time. After being reduced to 8mm x 5mm x 5mm, explants were transferred to modified MS media, which were kept in the dark for 10 days, followed by growth chamber at 28°C +/- 2°C with 16h light. Best results were obtained with 40mm x 20mm x 20mm or 40mm x 15mm x 15mm explants, immersed once or twice in 3.5% NaOCl. Higher contamination levels were observed 15 days after the beginning of the experiment; at 1% NaOCl, however, 96.67% contamination was observed after 3 days. Bacterial incidence was higher than fungal.

KEY-WORDS: Banana cv. Maçã; tissue culture; superficial sterilization; Musa AAB.

Downloads

Download data is not yet available.

Downloads

Published

2007-12-13

How to Cite

CARNEIRO, I. F.; ZICA, L. F.; CHAVES, L. J. COMPARISON AMONG DECONTAMINATION METHODS AT INITIAL PHASE OF BANANA IN VITRO CULTURE. Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics], Goiânia, v. 29, n. 2, p. 89–94, 2007. Disponível em: https://revistas.ufg.br/pat/article/view/2856. Acesso em: 29 nov. 2024.

Issue

v. 29, n. 2, Jul./Dec. 1999

Section

Research Article

License

Authors will not be paid for published articles and must waive their copyright in favor of the PAT journal. On the other hand, they are solely responsible for the content of those articles, even if the Editor holds the right to adjust them to the norms of the journal. Authors are allowed to publish their articles simultaneously in their institutional repositories, as soon as the original publication at the PAT journal is mentioned.