Imidazolium ionic liquid as potential contact lens disinfectant inactivating cystic resistance forms from ACANTHAMOEBA keratitis

Authors

  • Denise Leal dos Santos Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Setor de Parasitologia, Porto Alegre, RS, Brazil https://orcid.org/0000-0001-5761-1302
  • Beni Jequicene Mussengue Chaúque Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Setor de Parasitologia, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-9440-3227
  • Francisco Kercher Berté Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Setor de Parasitologia, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-4899-4820
  • Vinícius Demétrio da Silva Universidade Federal do Rio Grande do Sul (UFRGS), Institute of Chemistry, Laboratory of Technological Processes and Catalysis, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-7242-9442
  • Amanda Schmeling Franceschi Universidade Feevale, Instituto de Ciências da Saúde, Laboratório de Citotoxicidade, Campus II, Novo Hamburgo, RS, Brazil https://orcid.org/0000-0001-7575-3726
  • Diane Ruschel Marinho Hospital de Clínicas de Porto Alegre, Serviço de Oftalmologia, Departamento de Córnea, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-0050-5039
  • Sergio Kwitko Hospital de Clínicas de Porto Alegre, Serviço de Oftalmologia, Departamento de Córnea, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-0604-2776
  • Claudete Inês Locatelli Hospital de Clínicas de Porto Alegre, Serviço de Oftalmologia, Departamento de Córnea, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-8793-8194
  • Eduarda Correa Freitas Hospital de Clínicas de Porto Alegre, Serviço de Oftalmologia, Departamento de Córnea, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-0218-2296
  • Ana Luiza Ziulkoski Universidade Feevale, Instituto de Ciências da Saúde, Laboratório de Citotoxicidade, Campus II, Novo Hamburgo, RS, Brazil https://orcid.org/0000-0003-0850-4003
  • Henri Stephan Schrekker Universidade Federal do Rio Grande do Sul (UFRGS), Institute of Chemistry, Laboratory of Technological Processes and Catalysis, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-8173-3841
  • Marilise Brittes Rott Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Setor de Parasitologia, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-5227-4594

DOI:

https://doi.org/10.5216/rpt.v52i3.76409

Abstract

Keratitis caused by Acanthamoeba spp. is a rare disease, although increasingly common, especially among contact lens users. The occurrence and the devastating effect of this disease are associated with the lack of care in cleaning and disinfecting lenses and their storage cases, as well as ineffective drugs to mainly eliminate the parasite’s cysts. This work evaluated the amoebicidal activity of the imidazolium salt 1-hexadecyl-3-methylimidazolium chloride (C16MImCl) against cysts of two characterized isolates from Acanthamoeba keratitis cases (MZ404337 and MZ404332). The inactivation of 100% of the cysts was achieved at a concentration of 7.81 μg/mL for MZ404337 and of 1.95 μg/mL for MZ404332, both at 24 h and 48 h of exposure time. In contrast, a commercial formulation of chlorhexidine did not cause any reduction in the viability of the cysts. Related to the cytotoxicity to human HaCaT cells, C16MImCl is biocompatible at the concentration required to inactivate cysts. This shows that C16MImCl is a promising disinfectant for contact lenses and surfaces.

KEY WORDS: 1-hexadecyl-3-methylimidazolium chloride; Acanthamoeba keratitis; cysts; chlorhexidine; cytotoxicity.

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Author Biography

Beni Jequicene Mussengue Chaúque, Universidade Federal do Rio Grande do Sul (UFRGS), Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Setor de Parasitologia, Porto Alegre, RS, Brazil

and Universidade Rovuma, Center of Studies in Science and Technology (NECET), Biology Course, Niassa Branch, Lichinga, Mozambique.

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Published

2023-10-20

How to Cite

LEAL DOS SANTOS, D.; MUSSENGUE CHAÚQUE, B. J.; KERCHER BERTÉ, F.; DEMÉTRIO DA SILVA, V.; SCHMELING FRANCESCHI, A.; RUSCHEL MARINHO, D.; KWITKO, S.; LOCATELLI, C. I.; CORREA FREITAS, E.; ZIULKOSKI, A. L.; SCHREKKER, H. S.; ROTT, M. B. Imidazolium ionic liquid as potential contact lens disinfectant inactivating cystic resistance forms from ACANTHAMOEBA keratitis . Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 52, n. 3, p. 227–237, 2023. DOI: 10.5216/rpt.v52i3.76409. Disponível em: https://revistas.ufg.br/iptsp/article/view/76409. Acesso em: 22 dec. 2024.

Issue

Vol. 52 No. 3 (2023)

Section

ORIGINAL ARTICLES

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