A CELL-CULTURING SYSTEM FOR THE STUDY OF INTERACTION BETWEEN MACROPHAGES INFECTED WITH Leishmania amazonensis

Authors

  • Selma Giorgio Unicamp
  • Pedro Henrique Gallo-Francisco

DOI:

https://doi.org/10.5216/rpt.v48i2.58004

Abstract

The cell culture insert system is a culturing system for the study of contact-independent cellular communication. Leishmaniasis is a neglect tropical disease with no vaccines and the available
drugs present toxic side effects. Studies on Leishmania interaction with host macrophages aim to develop strategies for parasite control and drug development. The purpose of this study was to evaluate the effects of interaction between non-infected and L. amazonensis-infected human macrophages, by using the cell culture system. The results showed that the infection index was reduced by 56.2% as compared to controls only when infected macrophages were inserted on both sides of the Transwell membranes. An improvement in macrophage viability was also observed in this cell culture. The levels of interleukin-1?, an inflammatory cytokine, and nitric oxide, a microbicidal molecule, did not increase in L. amazonensis-infected macrophage
cultures in the Transwell system; thus other soluble factors were responsible for parasite control.

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Author Biographies

Selma Giorgio, Unicamp

Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo.

Pedro Henrique Gallo-Francisco

Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo.

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Published

2019-06-13

How to Cite

GIORGIO, S.; HENRIQUE GALLO-FRANCISCO, P. A CELL-CULTURING SYSTEM FOR THE STUDY OF INTERACTION BETWEEN MACROPHAGES INFECTED WITH Leishmania amazonensis. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 48, n. 2, p. 99–108, 2019. DOI: 10.5216/rpt.v48i2.58004. Disponível em: https://revistas.ufg.br/iptsp/article/view/58004. Acesso em: 16 aug. 2024.

Issue

Vol. 48 No. 2 (2019)

Section

ORIGINAL ARTICLES

License

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