A DOUBLE GAUZE FILTER MODIFICATION OF BAERMANN METHOD FOR ISOLATION OF Angiostrongylus costaricensis LARVAE FROM RODENT FECES

Authors

  • Ricardo Fontoura
  • Márcia Cristina Fernandes da Silva
  • Rafael Lucyk Maurer
  • Carlos Graeff-Teixeira

DOI:

https://doi.org/10.5216/rpt.v32i1.4357

Abstract

The Baermann method is a classical method for isolation of nematode larvae from either biological material or sou. The inclusion of a second gauze filter in the funnel resulted in a cleaner suspension of Angioslrongylus costaricensis fírst stage larvae (Li) from expenmentally infected rodent feces, without retention of LI as compared to the original method, if the preparation is left at rest for 12 hours. The second filter is a 2x2 cm gauze (4 layers) inserted in the opening of the straight portion of the funnel. A cleaner larvae suspension is important as a first step for purification and decontamination of the parasites to be cultivated in vitro.

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Published

2008-07-17

How to Cite

FONTOURA, R.; SILVA, M. C. F. da; MAURER, R. L.; GRAEFF-TEIXEIRA, C. A DOUBLE GAUZE FILTER MODIFICATION OF BAERMANN METHOD FOR ISOLATION OF Angiostrongylus costaricensis LARVAE FROM RODENT FECES. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 32, n. 1, 2008. DOI: 10.5216/rpt.v32i1.4357. Disponível em: https://revistas.ufg.br/iptsp/article/view/4357. Acesso em: 23 nov. 2024.

Issue

Vol. 32 No. 1 (2003)

Section

SHORT COMMUNICATION

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