PROFILE OF EXOANTIGENS FROM CLINICAL ISOLATES OF Paracoccidioides lutzii IN MATO GROSSO, BRAZIL

Authors

  • Daciene de Arruda Grossklaus
  • Doracilde Terumi Takahara
  • Cleoni Silvana Kruger
  • Andrea Maria Gonzaga
  • Zoilo Pires de Camargo
  • Andreia Ferreira Nery
  • Hugo Luciano Almeida
  • Rosane Christine Hahn

DOI:

https://doi.org/10.5216/rpt.v45i3.43514

Keywords:

Paracoccidioides lutzii, profiles, Mato Grosso.

Abstract

The objective of this study was to determine the immunochemical profile of three exoantigen preparations obtained from clinical samples of Paracoccidioides lutzii from the state of Mato Grosso, Brazil. The exoantigens were prepared according to a protocol standardized by Camargo et al. and their immunochemical profiles were analyzed using SDS-PAGE. The observed protein bands exhibited molecular weights of 20, 30, and 130 kDa.  The 130 kDa glycoprotein was detected in all three preparations. The detection of this high-molecular-weight protein suggests a distinct immunochemical profile. These differences may help to explain the false negative reactions obtained when using the antigen from P. brasiliensis reference strain B-339. This information is essential for increasing the specificity of serological tests used to monitor the prognosis of patients with paracoccidioidomycosis.

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Published

2016-09-30

How to Cite

DE ARRUDA GROSSKLAUS, D.; TERUMI TAKAHARA, D.; SILVANA KRUGER, C.; MARIA GONZAGA, A.; PIRES DE CAMARGO, Z.; FERREIRA NERY, A.; ALMEIDA, H. L.; HAHN, R. C. PROFILE OF EXOANTIGENS FROM CLINICAL ISOLATES OF Paracoccidioides lutzii IN MATO GROSSO, BRAZIL. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 45, n. 3, p. 265–272, 2016. DOI: 10.5216/rpt.v45i3.43514. Disponível em: https://revistas.ufg.br/iptsp/article/view/43514. Acesso em: 22 nov. 2024.

Issue

Vol. 45 No. 3 (2016)

Section

ORIGINAL ARTICLES

License

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