LARVICIDAL ACTIVITY OF 3-?-ACETOXYLABDAN-8(17)-13-DIEN-15-OIC ACID ISOLATED FROM THE MEDICINAL PLANT COPAIFERA RETICULATA AGAINST AEDES AEGYPTI EVIDENCED BY CHANGES MORPHOHISTOLOGIC.

Authors

  • Cleyde Ferreira Barreto Valotto Laboratório de Biologia, Fisiologia de Insetos/Bioatividade de Plantas, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, UFG, Goiânia, GO.
  • Gláucia Cavasin Departamento de Morfologia, Instituto de Ciências Biológicas, UFG, Goiânia, GO.
  • Heloisa Helena Garcia da Silva Laboratório de Biologia, Fisiologia de Insetos/Bioatividade de Plantas, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, UFG, Goiânia, GO.
  • Regina Geris Departamento de Química, Instituto de Química, Universidade Federal da Bahia, Salvador, BA
  • Ionizete Garcia da Silva Laboratório de Biologia, Fisiologia de Insetos/Bioatividade de Plantas, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, UFG, Goiânia, GO.

DOI:

https://doi.org/10.5216/rpt.v43i3.32214

Keywords:

Histology, morfology, dengue, yellow fever, natural products.

Abstract

The main mechanism of control of dengue has been through the fight against Aedes aegypti, the transmitter more competent. The fight is primarily to eliminate the potential breeding, health education and use of synthetic insecticides. These insecticides have performance but are highly toxic to vertebrates and the environment. With the emergence of resistance of mosquitoes to them, opens up an opportunity to insecticides of botanical origin. This study looks at the larvicidal activity of a solution of 3-?-acetoxylabdan-8(17)-13-dien-15-óico acid a solution of 3-?-acetoxylabdan-8(17)-13-dien-15-óico acid extracted from the medicinal plant Copaifera reticulata Ducke (Leguminosae) on the 3rd stage larvae of Ae. aegypti. Larvae were subjected to acid 9ppm for up to 24h. Those that reached lethargic state were collected and fixed in 4% in the paraformaldhyde buffer 0.1 M sodium cacodilate pH 7.2, included in resin and the slides stained with hematoxylin-eosin technique and examined by light microscope. This acid caused the death of the Ae. aegypti larvae by cell destruction in the midgut, by the high cytoplasmic vacuolation, cells and nuclei hypertrophy, degeneration of the edge brush, formation vesicular apical with release of cytoplasmic contents of cells, stratification of the epithelium and folds in the peritrophic matrix.

 

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Published

2014-10-09

How to Cite

VALOTTO, C. F. B.; CAVASIN, G.; DA SILVA, H. H. G.; GERIS, R.; DA SILVA, I. G. LARVICIDAL ACTIVITY OF 3-?-ACETOXYLABDAN-8(17)-13-DIEN-15-OIC ACID ISOLATED FROM THE MEDICINAL PLANT COPAIFERA RETICULATA AGAINST AEDES AEGYPTI EVIDENCED BY CHANGES MORPHOHISTOLOGIC. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 43, n. 3, p. 375–384, 2014. DOI: 10.5216/rpt.v43i3.32214. Disponível em: https://revistas.ufg.br/iptsp/article/view/32214. Acesso em: 21 nov. 2024.

Issue

Vol. 43 No. 3 (2014)

Section

ORIGINAL ARTICLES

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