EVALUATION OF SPECIFIC PRIMERS FOR SPECIES IDENTIFICATION OF Leishmania (V.) braziliensis

Authors

  • Lilian Mathias Marcussi
  • Cissiara Manetti Skraba
  • Tatiane França Perles
  • Raíssa Bocchi Pedroso
  • Maria Valdrinez Campana Lonardoni
  • Thaís Gomes Verzignassi Silveira

DOI:

https://doi.org/10.5216/rpt.v42i1.23595

Keywords:

Polymerase Chain Reaction, Leishmania braziliensis, Diagnosis.

Abstract

Leishmaniases are infectious diseases with different clinical forms and prognoses, making accurate species identification particularly important. We evaluated the performance of LBF1/LBR1 L. (V.) braziliensis specific primers by PCR and compared the results with Leishmania spp. identified by monoclonal antibodies (mAbs). Of 29 L. (V.) braziliensis identified by mAbs, 16 (53.3%) were detected; and 7 (63.6%) of 11 unidentified Leishmania spp. showed the 536 bp band. 87.7% of serodeme III Leishmania isolates were identified by these primers. These results indicate a poor correlation between the two identification methods used, and also suggest the existence of genetic variability among L. (V.) braziliensis isolates from the northwest region of Paraná state.

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How to Cite

MARCUSSI, L. M.; SKRABA, C. M.; PERLES, T. F.; PEDROSO, R. B.; LONARDONI, M. V. C.; SILVEIRA, T. G. V. EVALUATION OF SPECIFIC PRIMERS FOR SPECIES IDENTIFICATION OF Leishmania (V.) braziliensis. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 42, n. 1, 2013. DOI: 10.5216/rpt.v42i1.23595. Disponível em: https://revistas.ufg.br/iptsp/article/view/23595. Acesso em: 22 dec. 2024.

Issue

Vol. 42 No. 1 (2013)

Section

ORIGINAL ARTICLES

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