Detection of extended spectrum ?-lactamases (ESBLS) in isolates of uropathogenic Escherichia coli (UPECs) from patients in the community

Authors

  • Monique Santos do Carmo
  • Adriana de Mendonça Marques
  • Luis Henrique Bastos Gonçalves
  • Thiago Azevedo Feitosa Ferro
  • Cristina de Andrade Monteiro
  • Maria Rosa Quaresma Bomfim
  • Rosimary de Jesus Gomes Turri
  • Valério Monteiro-Neto
  • Patrícia de Maria Silva Figueiredo

DOI:

https://doi.org/10.5216/rpt.v41i4.21708

Keywords:

ESBL, UPEC, Disc approximation, PCR multiplex.

Abstract

The production of ESBL is an important mechanism of resistance to ?-lactam antimicrobials. The wide use of antibiotics in the treatment of urinary tract infections (UTI) has further aggravated this problem. Bacteria of the UPEC type are the main responsible for this kind of infection. Studies to understand the diversity of ESBL in Brazil are scarce and, in addition, many of them are directed to nosocomial samples. The objective of this study was to detect genes encoding ?-lactamases TEM, SHV and CTX-M through multiplex PCR, as well as to identify the isolates that produce them
through the disk approach method. From the total of isolates analyzed, 12.8% showed a positive phenotype for ESBL, while 65.8% amplified sequences homologous to the genes of ?-lactamases. Among these, the most prevalent were blaTEM and blaCTX-M, with 42.7% and 18.8%, respectively. Two isolates did not amplify for any genes in this study, despite of having expressed the enzyme in the phenotypic test. Based on to our results, we suggest the development of further studies aiming
at the molecular characterization to better elucidate the mechanism of spread of resistance among bacteria from patients in the community.

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How to Cite

CARMO, M. S. do; MARQUES, A. de M.; GONÇALVES, L. H. B.; FERRO, T. A. F.; MONTEIRO, C. de A.; BOMFIM, M. R. Q.; TURRI, R. de J. G.; MONTEIRO-NETO, V.; FIGUEIREDO, P. de M. S. Detection of extended spectrum ?-lactamases (ESBLS) in isolates of uropathogenic Escherichia coli (UPECs) from patients in the community. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 41, n. 4, 2012. DOI: 10.5216/rpt.v41i4.21708. Disponível em: https://revistas.ufg.br/iptsp/article/view/21708. Acesso em: 23 nov. 2024.

Issue

Vol. 41 No. 4 (2012)

Section

ORIGINAL ARTICLES

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