ELISA BASED ON RECOMBINANT MPB70 AND P27 FOR DETECTION OF ANTIBODIES AGAINST Mycobacterium bovis

Authors

  • Thaís A Farias
  • Flábio R Araújo
  • Ana Luíza AR Osório
  • Kláudia SG Jorge
  • Carlos NA Ramos
  • Ingrid IF Souza
  • Alexandre Azambuja
  • Cleber O Soares
  • Márcio R Silva
  • Aiesca O Pellegrin

DOI:

https://doi.org/10.5216/rpt.v41i2.19325

Keywords:

MPB70. p27, Mycobacterium bovis, Bovine tuberculosis.

Abstract

Bovine tuberculosis is a major disease caused by the bacterium Mycobacterium bovis. Skin tests and slaughter policies have reduced the incidence of bovine tuberculosis in many countries. However, more practical and efficient tools with high sensitivity and specificity values are needed. The aim of the present study was to develop an ELISA using the recombinant proteins MPB70 and p27 from M. bovis in order to detect antibodies against this bacterium in cattle. Sensitivity and specificity were respectively 88.7% and 94.6% for ELISA-MPB70 and 98.1% and 91.9% for ELISA-p27. The use
of a serological test such as ELISA with recombinant MPB70 and p27, together with cell tests, may solve some of the problems regarding the diagnosis of bovine tuberculosis, such as inconclusive results and the lack of detection in anergic animals in advanced stages of the disease.

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How to Cite

FARIAS, T. A.; ARAÚJO, F. R.; OSÓRIO, A. L. A.; JORGE, K. S.; RAMOS, C. N.; SOUZA, I. I.; AZAMBUJA, A.; SOARES, C. O.; SILVA, M. R.; PELLEGRIN, A. O. ELISA BASED ON RECOMBINANT MPB70 AND P27 FOR DETECTION OF ANTIBODIES AGAINST Mycobacterium bovis. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 41, n. 2, 2012. DOI: 10.5216/rpt.v41i2.19325. Disponível em: https://revistas.ufg.br/iptsp/article/view/19325. Acesso em: 23 nov. 2024.

Issue

Vol. 41 No. 2 (2012)

Section

ORIGINAL ARTICLES

License

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