Comparison of in house and commercial conjugates by rapid fluorescent focus inhibition test (RFFIT) in rabies antibodies evaluation

Authors

  • Wildeberg Cál Moreira
  • Wlamir Correa Moura
  • Marlon Vicente Silva
  • Rugimar Marcovistz

DOI:

https://doi.org/10.5216/rpt.v39i3.12207

Keywords:

Rabies virus, Serological tests, Rapid fluorescent focus inhibition test, Anti-rabies conjugate.

Abstract

Rabies is a lethal zoonosis that may be transmitted to man through virus inoculation,
mainly by the bite of infected animals. In 2005, the Brazilian Ministry of Health
spent about R$ 66 millions in epidemiological surveillance actions, in order to carry
out vaccination campaigns and acquisition of immunobiologicals. The serological
evaluation is the basic requirement for surveillance of individuals vaccinated.
Ninety one sera, from 34 vaccinees, were selected to evaluate the antibody titration
by rapid fluorescent focus inhibition test adapted to 96-well microplates, to compare
an in house produced conjugate and a commercial one. Seventy four sera (82.2%)
had titers of ?0.5 IU/mL and 12 sera (13.3%) had titers of <0.5 IU/mL. Theses
results showed that the rapid fluorescent focus inhibition test using the in house
conjugate was as sensitive as the commercial one. The difference between the
results using both conjugates was not significant; the antibody titers were highly
correlated (r= 0.94).

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Published

2010-10-21

How to Cite

MOREIRA, W. C.; MOURA, W. C.; SILVA, M. V.; MARCOVISTZ, R. Comparison of in house and commercial conjugates by rapid fluorescent focus inhibition test (RFFIT) in rabies antibodies evaluation. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 39, n. 3, p. 163–172, 2010. DOI: 10.5216/rpt.v39i3.12207. Disponível em: https://revistas.ufg.br/iptsp/article/view/12207. Acesso em: 18 may. 2024.

Issue

Vol. 39 No. 3 (2010)

Section

ORIGINAL ARTICLES

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