RESISTANCE TO HEAVY METALS, ANTIMICROBIAL AND BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM BEACHES OF SANT LUIS - MARANHÃO

Authors

  • André Luiz Raposo Barros Mestrando em Agroecologia, Universidade Estadual do Maranhão (UEMA), MA.
  • Márcio Fernandes Alves Leite Doutorando em Agroecologia, Universidade Estadual do Maranhão (UEMA), MA.
  • Ronildson Lima Luz Doutorando em Agroecologia, Universidade Estadual do Maranhão (UEMA), MA.
  • Nathalia Cunha Almeida Pinheiro Mestranda em Sustentabilidade de Ecossistemas, Universidade Federal do Maranhão (UFMA), MA.
  • Monique Santos do Carmo Mestranda em Ciências da Saúde, Universidade Federal do Maranhão (UFMA), MA.
  • Flávio Henrique Reis Moraes Professores da Universidade CEUMA, MA.
  • Patrícia de Maria Silva Figueiredo Professores da Universidade CEUMA, MA.

DOI:

https://doi.org/10.5216/rpt.v43i3.32213

Keywords:

Coliforms, antibiogram, metals heavy, Public Health, biolfilms

Abstract

The city of São Luís located in the State of Maranhão has inefficient treatment of their effluent, released into the rivers or directly on the beaches. This study evaluated the resistance of 39 strains of E. coli to Cu, Ni, Cd e Hg, their susceptibility to antibiotics and ability of biofilm production. Samples were collected from the beaches of Ponta D'Areia, São Marcos, Calhau and Olho d’Água. The antibiotic susceptibility was performed by disk diffusion method with the following antibiotics: Aztreonam (ATM), Imipenem (IMP), Cephalothin (KF); Ampicillin / Sulbactam (ASB), Gentamicin (CN), Ampicillin (AMP); Cefuroxime Sodium (CXM), Amoxicillin (AML); Piperacillin Tazobactam (TZP), Trimethoprim-sulfamethoxazole (SXT), Amoxicillin + clavulanic acid (AMC). The detection of biofilms production were performed in Congo Red Agar (CRA) and spectrophotometry (ESP). The results showed 2 strains Hg tolerant (1 mM), 9 strains resistant to Cu and Cd (4 mM) and 12 strains resitente to Ni (4 mM). The antibiotic susceptibility tests showed resistance to ATM (15% of samples), for KF (95%), BSA (85%), CN (5%), AMP (87%), CXM (13%), TZP (5%) SXT (74%) and AMC (18%). For the synthesis of biofilm in CRA 20% showed strong production, 36% low and 44% did not produce. By spectrophotometry only 1 (2.5%) proved to be moderate biofilm producer, 5 (12.5%) were classified as non-producers and other 34 (85%) weak produce. The presence of bacteria with antimicrobial and heavy metal resistance profile, and production of biofilms represents a public health risk and direct changes in ecological communities of marine microorganisms.

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Published

2014-10-09

How to Cite

BARROS, A. L. R.; LEITE, M. F. A.; LUZ, R. L.; PINHEIRO, N. C. A.; DO CARMO, M. S.; MORAES, F. H. R.; FIGUEIREDO, P. de M. S. RESISTANCE TO HEAVY METALS, ANTIMICROBIAL AND BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM BEACHES OF SANT LUIS - MARANHÃO. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 43, n. 3, p. 277–289, 2014. DOI: 10.5216/rpt.v43i3.32213. Disponível em: https://revistas.ufg.br/iptsp/article/view/32213. Acesso em: 22 may. 2024.

Issue

Vol. 43 No. 3 (2014)

Section

ORIGINAL ARTICLES

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