TFC035 Influence of dendrimers in the subcellular localization of protoporphyrin IX in carcinoma cells for Photodynamic Therapy

Abstract

The greater efficacy of photodynamic therapy (PDT) depends on the selective accumulation of the photosensitizer in the cells mitochondria. Protoporphyrin IX (PpIX) is an efficient photosensitizer when administered in the form of its non-photosensitizer precursor, the 5-aminolevulinic acid. However, when administered directly, PpIX high hidrophobicity and diffuse distribution into the cells impaired PDT efficacy. Target PpIX to the cells mitochondria could improve PDT efficiency with this drug. This target delivery can be achieved with the use of drug delivery systems, such as the dendrimers. Polyamidoamine generation – 4 hydroxyl terminated dendrimer (PAMAM G4-OH) are hyberbranched polymers capable to form complexes with PpIX, increasing PpIX aqueous solubility (Patente BR 10 2012 002494 2 (2012)) and likely changing its inner cell distribution.