PREGNANCY RATES IN HEIFERS INSEMINATED WITH SEMEN OF NELORE BULLS SEXED BY FLOW CYTOMETRY
DOI:
https://doi.org/10.5216/cab.v8i3.1734Keywords:
Pregnancy, Insemination, ReproductionAbstract
The flow-sorting sperm technology is considered as the most promising technique for sexing semen, due to the separation accuracy of around 90%. Thus, the objec-tive of this study was to determinate the effect of Articial Insemination (AI) with semen of Nelore bulls sexed by flow cytometry on the pregnancy rate and its accuracy. Throughout the study, 427 Nelore heifers were inseminated with only one dose of semen as follows: 241 heifers were treated with flow-sexed sperm (3 x 106 total flow-sorting X-chromosome bearing sperm/dose) and 181 were treated with unsexed sperm (10 x 106 total sperm/dose). The heifers were inseminated after detection of natural heat or after syn-chronization treatment. The results obtained for pregnancy rates did not indicate differences between flow-sexed and unsexed sperm and the accuracy in the sex determination presented high levels and reliability (92.4%).
KEY-WORDS: Bovine, Nelore, spermatozoa, sexing.
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