EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)
DOI:
https://doi.org/10.5216/cab.v10i4.1200Keywords:
Animal sanityAbstract
The objective of this study was to evaluate the effect of three disaccharides (sucrose, threalose and lactose) used as nonpenetrating cryoprotectants in extender for mice semen. The parameters evaluated were sperm motility (MOT) and cleavage rate (CLV) after in vitro fertilization in the SWISS-ALBINA and BALB/c lines. The treatments were S1=sucrose; S2=threalose; S3=lactose, for SWISS-ALBINA; and B1=lactose; B2=threalose for BALB/c. MOT was evaluated after: thawing (THA), centrifugation and re-suspension in P-1 medium (CEN) and after 10 minutes of incubation (10M). The MOT for the SWISS-ALBINA line was higher for S2 (P<0.001) in the 3 evaluated steps (47% at DES; 66.5% at CEN and 67.2% at 10M) than for S1 (32.5% at DES; 51.5% at CEN and 47.7% at 10M) and S3 (30% at DES, 46.5% at CEN and 32.7% at 10M). For the BALB/c line, MOT was superior for B2 than for B1 (P<0.001). Thus, the tested disaccharides, especially threalose, can be recommended for freezing of mice sperm.KEY WORDS: Cryopreservation, disaccharides, mice, semen.
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Published
2009-12-17
How to Cite
PIASSI, L. M.; CORCINI, C. D.; CURCIO, B. da R.; PANZARDI, A.; MARTINS, N. O.; AMADO, M. O.; VARELA JUNIOR, A. S.; LUCIA JR, T.; DESCHAMPS, J. C. EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 4, p. 1161–1170, 2009. DOI: 10.5216/cab.v10i4.1200. Disponível em: https://revistas.ufg.br/vet/article/view/1200. Acesso em: 23 nov. 2024.
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Veterinary Medicine
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