Antimicrobial resistance in bacteria isolated from environmental surfaces in a municipal dog shelter: a One Health approach

Willian Fontini Marangon; Francieli Bonato; Halison Murilo Oliveira; Kariny Aparecida Rúbio Espessato; Jorge de Azevedo; Henrique Susumu Tanaka; Isabela Carvalho dos Santos; Heloiza de Oliveira Marchi; Vitor Yukio Takahashi; Regiane Pereira Baptista da Silva; Lidiane Nunes Barbosa; Daniela Dib Gonçalves

doi:10.1590/1809-6891v27e-86001E

Autores

Willian Fontini Marangon Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0002-4646-0147
Francieli Bonato Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0003-3235-5124
Halison Murilo Oliveira Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0009-0007-1269-1506
Kariny Aparecida Rúbio Espessato Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0002-5243-2986
Jorge de Azevedo Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0009-0008-8229-1871
Henrique Susumu Tanaka Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0009-0007-2312-0610
Isabela Carvalho dos Santos Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0002-7971-5126
Heloiza de Oliveira Marchi Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0009-0008-8076-2852
Vitor Yukio Takahashi Abrigo Municipal de Animais, Umuarama, Paraná, Brasil https://orcid.org/0009-0002-7493-0350
Regiane Pereira Baptista da Silva Abrigo Municipal de Animais, Umuarama, Paraná, Brasil https://orcid.org/0000-0002-5068-8355
Lidiane Nunes Barbosa Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0001-5762-8091
Daniela Dib Gonçalves Universidade Paranaense (Unipar), Umuarama, Paraná, Brasil https://orcid.org/0000-0001-8322-8905

DOI:

https://doi.org/10.1590/1809-6891v27e-86001E

Resumo

Ambientes compartilhados podem atuar como reservatórios de bactérias resistentes, favorecendo sua disseminação entre espécies e representando risco à saúde pública. Abrigos de cães, frequentemente com alta densidade populacional e infraestrutura sanitária limitada, podem favorecer a persistência e circulação desses microrganismos. O objetivo deste estudo foi avaliar o perfil de resistência antimicrobiana de bactérias presentes em superfícies ambientais de um abrigo municipal de cães no noroeste do Paraná, Brasil. Foram coletadas 60 amostras de superfícies frequentemente manipuladas, utilizando swabs estéreis. As amostras foram cultivadas em caldo Brain Heart Infusion e posteriormente semeadas em ágar sangue para isolamento deStaphylococcus spp. e enterobactérias. A identificação foi realizada por características morfológicas e testes bioquímicos, enquanto a resistência antimicrobiana foi determinada pelo teste de disco difusão. Isolados resistentes à oxacilina e cefoxitina foram submetidos à PCR para detecção do gene mecA. Das amostras analisadas, 45 % foram identificadas como Staphylococcus spp. e 11 % como enterobactérias. A maioria dos estafilococos apresentou elevada resistência a diversos antimicrobianos, com 88,9 % classificados como multirresistentes. Nenhum isolado apresentou o gene mecA. Os resultados evidenciam a presença de bactérias multirresistentes em superfícies do abrigo, indicando potencial risco de disseminação entre animais, humanos e ambiente.
Palavras-chave: Abrigo de cães; enterobactérias; estafilococos; multirresistência; Saúde Pública.

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