TRYPANOSOMA spp. in captive primates in a brazilian zoo

Autores

  • Wesley Jose dos Santos São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0001-8042-5524
  • Livia Maisa Guiraldi São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0002-1690-1693
  • Mirian dos Santos Paixão Marques São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0001-9060-2424
  • Maria Fernanda Alves-Martin São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0001-9558-646X
  • Gabriela Pacheco Sanchez University of São Paulo (USP), Veterinary Medical School, Department of Preventive Veterinary Medicine and Animal Health, São Paulo, São Paulo, Brazil https://orcid.org/0000-0002-0394-0589
  • Daniela Barbosa da Silva São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, São Paulo, Brazil https://orcid.org/0000-0001-6910-7058
  • Virginia Bodelao Richini-Pereira São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0001-8312-7397
  • Cilmery Suemi Kurokawa ão Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil https://orcid.org/0000-0003-1380-7527
  • Simone Baldini Lucheis São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

DOI:

https://doi.org/10.5216/rpt.v50i2.69303

Resumo

Captive animals, despite the constant care provided, are susceptible to infections from different sources. We herein report the natural trypanosome infection of 11 (28.2% positive) out of 39 non-human primates from 13 different species, in a Brazilian zoological park. Immunofluorescent antibody test (IFAT) and conventional polymerase chain reaction (cPCR) ruled out Trypanosoma cruzi, the etiological agent of Chagas disease. However, sequencing performed with positive samples employing hsp70 primers revealed similarities from 86% to 88% to diverse trypanosomes, including T. cruzi, Trypanosoma grayi, Trypanosoma lewisi, Trypanosoma rangeli and Trypanosoma vivax. We believe that the low similarity values obtained by sequencing reflect the difficulties in the molecular identification of trypanosomes, which share a large portion of their genetic material; this similarity may also preclude the diagnosis of co-infection by more than one trypanosome species. Thus, our study demonstrates the presence of diverse trypanosomes in primates, which are susceptible to infection by these parasites. Mechanical devices such as windows and bed nets, etc., are required to avoid vector insects in these environments, in addition to preventive quarantining of animals recently introduced into zoos. Therefore, investigation of the parasites in both the animals already residing in the zoo and those being introduced is of paramount importance, although no easy task.

KEY WORDS: Non-human primates; monkey; diagnosis; trypanosomes.

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Biografia do Autor

Virginia Bodelao Richini-Pereira, São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

1.São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

2. Adolfo Lutz Institute, Regional Laboratories Center, Bauru, São Paulo, Brazil

Cilmery Suemi Kurokawa, ão Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

1. São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

2. São Paulo State University (UNESP), Medical School, Department of Pediatrics, Botucatu, São Paulo, Brazil

 

Simone Baldini Lucheis, São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

1. São Paulo State University (UNESP), Medical School, Graduate Course in Tropical Diseases, Botucatu, São Paulo, Brazil

2. São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Department of Animal Production and Preventive Veterinary Medicine, Botucatu, São Paulo, Brazil

3. Paulista Agency of Agribusiness Technology (APTA), Bauru, São Paulo, Brazil

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Publicado

14-06-2021

Como Citar

Jose dos Santos, W., Maisa Guiraldi, L., dos Santos Paixão Marques, M., Fernanda Alves-Martin, M., Pacheco Sanchez, G., Barbosa da Silva, D., Bodelao Richini-Pereira, V., Suemi Kurokawa, C., & Baldini Lucheis, S. (2021). TRYPANOSOMA spp. in captive primates in a brazilian zoo. Revista De Patologia Tropical / Journal of Tropical Pathology, 50(2), 121–134. https://doi.org/10.5216/rpt.v50i2.69303

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Seção

ARTIGOS ORIGINAIS / ORIGINAL ARTICLES