DETECTION OF SALIVIRUS IN RAW SEWAGE SAMPLES IN RIO DE JANEIRO, BRAZIL

  • Marcelle Silva-Sales Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, Brazil
  • Mariana Seglia Caldas Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil
  • Julia Monassa Fioretti Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil
  • Monica Simoes Rocha Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.
  • Tulio Machado Fumian Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.
  • Marize Pereira Miagostovich Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil

Abstract

Gastroenteric viruses are important pathogens related to cases of acute gastroenteritis, affecting millions of people worldwide with a major impact on children under five in developing countries. The introduction of metagenomic approach techniques in the 2000s has allowed the description of new viruses, among them Salivirus, which has been associated worldwide with cases of diarrhea. This study aimed to detect salivirus in raw sewage samples from a wastewater treatment plant (WWTP) collected between June 2013 and May 2014 in Rio de Janeiro, Brazil. Fifty-two samples collected weekly were tested by using a real-time quantitative PCR (qPCR).  Salivirus genome was detected in 71.1% (37/52) of the samples, with viral concentration ranging from 7.56 x 104 to 7.20 x 106 genomic copies per liter. Higher viral loads were detected in the summer and fall of 2014, although these data were not sufficient to infer seasonality for this virus. The high prevalence of salivirus in sewage samples highlights the importance of viral research in wastewater to generate data on salivirus circulation, increasing understanding regarding its dissemination in the population.
KEY WORDS: Wastewater; viral detection; salivirus; Brazil; raw sewage; qPCR.

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Author Biographies

Marcelle Silva-Sales, Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, Brazil

1. Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.
2. Laboratory of Virology and Cell Culture, Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, Brazil

Mariana Seglia Caldas, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Julia Monassa Fioretti, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Monica Simoes Rocha, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Tulio Machado Fumian, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Marize Pereira Miagostovich, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Ministry of Health, Brazil.

Published
27-05-2020
How to Cite
Silva-Sales, M., Seglia Caldas, M., Monassa Fioretti, J., Simoes Rocha, M., Machado Fumian, T., & Pereira Miagostovich, M. (2020). DETECTION OF SALIVIRUS IN RAW SEWAGE SAMPLES IN RIO DE JANEIRO, BRAZIL. Revista De Patologia Tropical / Journal of Tropical Pathology, 49(2), 73-78. https://doi.org/10.5216/rpt.v49i2.63619
Section
ARTIGOS ORIGINAIS / ORIGINAL ARTICLES