In vitro EFFECTS OF SODIUM SELENITE SUPPLEMENTATION ON CELL VIABILITY OF DIFFERENT FORMS OF Trypanosoma cruzi

  • Otavio Henrique Thiemann University of São Paulo, Brazil
  • Marco Tulio Alves da Silva University of São Paulo, Brazil
  • Gisele Bulhoes Portapilla School of Pharmaceutical Sciences of Ribeirão Preto, SP, Brazil
  • Alan Raphael de Farias Klein Moraes University of São Paulo, Brazil

Abstract

Selenium is an essential trace element which, at adequate levels, presents different beneficial biological effects, such as cancer regression, tissue development and protection against oxidative damage. The positive effects of this element are related to the expression of selenoproteins and their ability to modulate the immune system and the oxidative stress response. In Chagas disease and sleeping sickness, selenium supplementation has shown blood parasitism reduction and the alleviation of specific aspects of the diseases, such as diminishing anemia in sleeping sickness or minimization of myocardial and right ventricular chamber damage in Chagas
disease. Although the influence of selenium in trypanosomiasis has been investigated, the direct effects of sodium selenite supplementation on trypanosome cells are poorly understood. Treatment of Trypanosoma cruzi cultures with low selenium doses demonstrated different results, according to the parasite evolutive form analyzed. Epimastigote cultures supplemented with 100 nM of sodium selenite presented cell growth increment, which varies from 10 to 40% according to the parasite strain assayed. Selenium concentration around 600nM leads to a 30% increase in the amastigote form number, whereas, at the same dose, the mammal host cell presented no cellular growth alteration. For the bloodstream form, the results agree with the
literature, and all sodium selenite concentrations tested, demonstrated a reduction in parasite viability. The data suggest that selenium supplementation, under specific conditions, could increase T. cruzi viability, demonstrating that a strategy for using selenium as an adjuvant in Chagas disease treatment requires additional experimentation. 
KEY WORDS: Selenium; trypanosomiasis; Chagas disease; Trypanosoma cruzi.

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Author Biographies

Otavio Henrique Thiemann, University of São Paulo, Brazil

1. São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, Brazil.
2. Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, Brazil.

Marco Tulio Alves da Silva, University of São Paulo, Brazil

São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, Brazil.

Gisele Bulhoes Portapilla, School of Pharmaceutical Sciences of Ribeirão Preto, SP, Brazil

School of Pharmaceutical Sciences of Ribeirão Preto, (USP), Ribeirão Preto, Brazil.

Alan Raphael de Farias Klein Moraes, University of São Paulo, Brazil

1. São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, Brazil.
2. Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, Brazil.

Published
27-05-2020
How to Cite
Henrique Thiemann, O., Tulio Alves da Silva, M., Bulhoes Portapilla, G., & Raphael de Farias Klein Moraes, A. (2020). In vitro EFFECTS OF SODIUM SELENITE SUPPLEMENTATION ON CELL VIABILITY OF DIFFERENT FORMS OF Trypanosoma cruzi. Revista De Patologia Tropical / Journal of Tropical Pathology, 49(2), 120-130. https://doi.org/10.5216/rpt.v49i2.61410
Section
ARTIGOS ORIGINAIS / ORIGINAL ARTICLES