SCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KIT

Autores

  • Hugo César Pereira Santos
  • Larissa Córdova Turones
  • Fabíola Souza Fiaccadori
  • Divina das Dôres de Paula Cardoso
  • Menira Borges de Lima Dias e Souza

DOI:

https://doi.org/10.5216/rpt.v43i2.31111

Palavras-chave:

Asymptomatic children, enzyme immunoassay, Norovirus, sensitivity, specificity

Resumo

Norovirus is the leading cause of non-bacterial acute gastroenteritis outbreaks worldwide. Recently, third generation Enzyme Immunoassay (EIA) commercial kits have been developed, and controversial results have been obtained by different studies regarding the sensitivity and specificity of these assays. Therefore, the aim of this study was to test 60 fecal samples, previously tested as positive by RT-PCR for caliciviruses (40 norovirus-positive and 20 sapovirus-positive samples), for qualitative determination of genogroup I and II noroviruses by a commercial EIA kit (RIDASCREEN® Norovirus (C1401) 3rd Generation, R-Biopharm, Darmstadt, Germany). The samples were obtained from 30 children aged less than five years, mostly asymptomatic, who attend a day-care center in Goiânia, Goiás, Brazil. The results conferred a positivity rate for NoV of 35% and a specificity rate of 100% for the EIA, when compared to the RT-PCR. The test also failed to detect samples that were positive for GI.1 and GI.4 norovirus. The presumably lower viral load of asymptomatic children might be related to the poor sensitivity. Our results reinforce the notion that
screening of samples by molecular assays, especially of samples that might have a low number of viral particles such as those obtained from asymptomatic patients, should not be replaced by the use of EIA kits.

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Publicado

2014-07-16

Como Citar

PEREIRA SANTOS, H. C.; TURONES, L. C.; FIACCADORI, F. S.; PAULA CARDOSO, D. das D. de; DIAS E SOUZA, M. B. de L. SCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KIT. Revista de Patologia Tropical / Journal of Tropical Pathology, Goiânia, v. 43, n. 2, p. 143–149, 2014. DOI: 10.5216/rpt.v43i2.31111. Disponível em: https://revistas.ufg.br/iptsp/article/view/31111. Acesso em: 14 out. 2024.

Edição

Seção

ARTIGOS ORIGINAIS / ORIGINAL ARTICLES